antYbuddY.com By Giancarlo Barone

6.4% funded

£10,000.00 the goal

Closed

£641.66 pledged so far from 19 backers
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Latest Backers

  • Helen Garritt : £100.00
  • Anonymous : £5.00
  • Rachael Dunlop : £16.45
  • Deborah Marsh : £62.60
  • Anonymous : £12.36
  • Kehinde Ross : £20.00
  • Benjamin Martin : £15.00
  • william eaton : £10.00
  • Dominique Laugel : £26.46
  • Anonymous : £50.00
  • Kasper van Gelderen : £8.79
  • Luke McKenzie : £10.00
  • Amy Ricketts : £100.00
  • Lynda ricketts : £100.00
  • Susan Nasif : £15.00
  • Spencer Collis : £25.00
  • Thomas Jones : £25.00
  • Susan Duncan : £10.00
  • David King : £30.00

The antibody sellers’ market is self-regulating and currently lacks standardised quality control. Poor quality antibodies that are not fit for purpose are being sold to the scientific community for use in experimental research. This has resulted in the waste of valuable research time and a reported $800 million per annum but the exact amount is likely to be considerably more. An antibody review website that only hosts 'High Quality' antibody reviews with an accompanying peer-to-peer support forum would help the scientific community negate unnecessary waste on poor quality antibodies. 

Opens 4 October 2016

A BRIEF INTRODUCTION

I am a postdoctoral research scientist with twelve years experience in the field of cancer research. Throughout that time I have observed a significant waste of research time and funding. It is no doubt a problem shared by research scientists, clinical researchers and PhD students worldwide.

Scientists purchase products to carry out experiments from specialised companies and distributors. Certain scientific products do not come with guarantees, lack specific experimental protocols and have rarely been validated. Instead It's left to the individual scientist to spend valuable resources in seeking to ascertain suitability and specific experimental conditions.

The antibody market is a notable minefield. Scientists, clinical researchers and histologists use antibodies in multiple applications. They are an integral component used in cancer and human disease research.

A SOLUTION TO THE PROBLEM

The antibody market is substantial and currently worth over $2.6 billion per annum globally. There exists over 1 million antibodies for use in research applied to different types of organisms.

Each antibody costs approximately £300 and comes with no guarantee. Indeed, all too often antibodies are either useless or work poorly in one application but good in another. Scientists spend valuable resources identifying specific experimental conditions for each antibody in each application. 

Surprisingly, most antibodies have never been validated. This means that until the end user spends further resources validating the antibody, they can't be sure the antibody is recognising its intended target. In this scenario the end user would get misleading experimental results. 

THE WEBSITE

A Worldwide Problem That Requires a Worldwide Solution 

I will establish a free to use antibody review website with a peer-to-peer support forum for the worldwide scientific community to share. I want the scientific community to purchase antibodies with confidence!

The review website, antYbuddY.com, will focus on the antibody market but will also review other popular products for use in experiments such as siRNA technology, emerging Crispr technology and ready to use kits. 

antYbuddY.com will invite the scientific community to submit reviews on antibodies they have used. All submissions will provide product details with an outline of experimental conditions and will always require supporting experimental data.

antYbuddY.com will always provide the scientific community with reviews they can trust. Therefore, reaching a 'High Quality' for each review is paramount. 

Each review will always provide the following information:

  • Comprehensive product details: including manufacturer, product code, species re-activity, clonality and batch number.
  • Experimental settings: including antibody concentration, antibody diluent, incubation period and temperature.
  • All reviews must include supporting scientific data: one figure for each application used such as WB, IP, IF and IHC.
  • Every review submission will ask if the antibody has been validated via knockdown / depletion: If answered yes, this must be supported. by a figure for each application, as above.
  • Validated antibodies will be given a clear highlighted status.
  • Send the reviewer a message regarding their review (opt-in only).

WEBSITE FORUM, PROTOCOLS DEPOSIT BOX AND VLOGS

Online Forum

The forum will provide a platform connecting the scientific community and encouraging peer-to-peer support. It will provide support and advice with any issues you are encountering in the laboratory from your peers.

The forum will be a valuable platform for the scientific community and will help scientists optimise experiments, impacting on the temporal and financial costs of their projects. 

Protocols Deposit Box

Researchers will be encouraged to upload useful experimental protocols into a 'Protocols Deposit Box' for their peers to view and download

VLOGS

Every month antYbuddY.com will invite individuals from the scientific community to make a series of short videos to host on antYbuddy.com.

antYbuddY.com will invite junior and established senior scientists from the scientific community to offer advice, tips, reviews and experiences on wide ranging topics.

The estimated project timescale is 3 months from funding.

  • Design phase (1 month): not only visual design, but also information architecture, copy writing, and so on.
  • Technical prototyping phase (1 month): this is where the static designs are built out into a working website.
  • Closed Beta phase (1 month): during this time, a small group of users will be let loose on the site with the goal of testing it as much as possible before it goes public. All feedback will be incorporated into the next release.
  • Public Beta launch!

FUNDING & COSTS

The web design and development costs are £16,000. £8000 is the minimal funds required to build a bespoke review website with the required functionality that antYbuddY.com needs. The remaining funds will go towards hosting and maintaining antYbuddY.com in the first several months. 

Long Term Sustainability

To keep antYbuddY.com running in the long term it needs to be self-sufficient. This can only be achieved through two avenues given that I want the website to be free to all users.

Users could be asked to donate to the cost of running the site which would be a risky and insecure prospect. Realistically the long term future of antYbuddY.com would be best served allowing selective and relevant advertising. With this, selected advertisers will be invited onto the website. This will ensure the long term future of antYbuddY.com and potentially fund further development of antYbuddy.com giving a better website experience for the scientific community.

Thank you and small reward for supporting  my campaign!

A BIG thank you to everyone for your support. You will be invited to the websites "Early Access Closed Beta" version to help the development team test the website before its launch! In addition, a dedicated webpage on antYbuddY.com will be created for you where your name will take permanent presence together with my thanks.

£5 or more

Thank you! You’ll get a personal shout out from me

£15 or more

10 % discount* off a full size Novus Antibody and a freebie1 of your choice (pens, flash drives, luggage tags and seed packets).

£25 or more

20 % discount* off a full size Novus Antibody and a freebie1 of your choice (pens, flash drives, luggage tags and seed packets)

£30 or more

£10 amazon voucher, 20 % discount* off a full size Novus Antibody and a freebie1 of your choice (pens, flash drives, luggage tags and seed packets)

£100 or more,

You will have your photo added as a permanent fixture, on a dedicated webpage on http://antybuddy.com), £10 amazon voucher, 20% discount* off full size Novus antibody and a freebie of your choice (pens, flash drives, luggage tags and seed packets)

£3000 (limited to 2 spaces2)

Dedicated space (logo/banner) on the AntyBuddy website for 3 months after beta testing

£5000 (limited to 1 space2)

Dedicated space (logo/banner) on the AntyBuddy website for 6 months after beta testing

£8000 (limited to 1 space2)

Dedicated space (logo/banner) on the AntyBuddy website for 12 months after beta testing

 

*Eligible countries: US, UK, Ireland, Germany, France, Switzerland, Italy, Denmark, Finland, Sweden, Norway, Iceland and the Netherlands

  1. Due to postage restrictions, Freebies are limited to UK residents only. First come first served basis as stock is limited
  2. Preference given based on registration date of donor

A Waste of Valuable Resources 

Significant research funding and time is wasted because of poor quality antibodies and a lack of quality worthwhile resources like an antibody review website and peer-to-peer support forum, that assists scientists make well informed purchases.

Consider that an average sized laboratory readily purchases £20,000 - £30,000 on antibodies over a 5 year funding period. A lot of which don't work efficiently if at all and have never been adequately validated. With this, It's easy to grasp that many research hours and £millions in research funds are squandered every year just on the antibody market alone. That's charitable funds donated by me and you or taxpayers money distributed through research councils from governments.

A reliable resource that equips the scientific community, allowing them to make an informed decision on antibody and product purchasing is greatly needed. 

I will create a website for the scientific community which will help them make an informed choice on their antibody purchases based on credible reviews and backed up with real scientific evidence. 

Several links that highlight the clear need for a solution: 

In addition, I kindly invited scientists from several UK research departments to answer a few pertinent questions in an anonymous survey. The results of which are below:

https://www.surveymonkey.com/results/SM-7HBGS8WW/

 

 

This campaign is driven by my passion to create a modern open access resource that supports the worldwide scientific community in reducing laboratory costs made through better purchases and to carry out experiments in a timelier manner.

The success of antybuddy.com will not only increase the standard of experimental research but more importantly help reduce the unnecessary waste of research funds that charities and governments invest in bioresearch.

In addition, the existence of independent resources like antybuddy.com will provide pressure on antibody sellers to produce better quality products for the scientific community which can only be a good thing. 

Dr. Spencer J. Collis, Reader in Genome Stability, University of Sheffield Medical School “ As both a researcher in the laboratory, and as a group leader, I have experienced the frustration with having to purchase unvalidated antibodies that have yielded unreliable and unusable results. Each pot of antibody costs a research laboratory around £250-300, with such funds coming from grants funded by either charities or government money. Having validated antibodies could therefore reduce wasted costs like this and saving a research laboratory thousands of pounds, which can be used for other aspects of the research project, and help progress the work in a timely manner.”

Dr. Chris Staples, Research Lecturer in Cancer Genetics and Genome Instability, Bangor University  “Like countless other people, I have wasted thousands of pounds on poor antibodies. antYbuddY.com will be nothing short of a godsend!”

Dr. David King, Clinical lecturer in paediatrics and PhD student in molecular oncology. “Antibodies are an essential tool in science but all too often they don't work properly. This can result in many months of wasted time, effort and money. The website suggested could go a long way to resolving these issues and would be a huge benefit to the entire scientific community.”

 

Dr Giancarlo Barone

I completed my BSc in Human Genetics at the University of Leeds. I then moved to Cambridge for one year to work as a research assistant at the Babraham Institute, researching the contribution of a fusion protein, NPM-ALK, in Non Hodgkin Lymphoma.

Following my time in Cambridge I moved to Birmingham where I completed my Ph.D in Cancer Studies at the University of Birmingham, researching the field of DNA damage signalling and Repair.

Having successfully completed my Ph.D I gained my first post-doctoral position working in the Department of Biochemistry at the University of Leicester while researching kinases involved in cell division, centrosome biology and genome stability.

I am currently working in the Department of Oncology and Metabolism at the University of Sheffield investigating uncharacterised genes that play a role in genome stability, DNA repair or repliaction stress signalling.

Researchgate Profile:  https://www.researchgate.net/profile/Giancarlo_Barone2

Linkdin Profile:  https://www.linkedin.com/in/giancarlo-barone-07913311b?trk=hp-identity-name

Twitter: https://twitter.com/Anty_Buddy

Facebook: https://www.facebook.com/AntyBuddy-574434542704115/

 

Mark Bain: Web Design and Development

Mark Bain: Web Design and Development

The design and development of antYbuddY.com will be organised by Mark Bain. His profile link and project timeline are below:

http://markbaindesign.com

"I'm excited about working on the antYbuddY website, which strikes me as an incredibly worthwhile project that will greatly improve the effectiveness of research".    

 

 

Rewards

A BIG thank you to everyone for your support. You will be invited to the websites "Early Access Closed Beta" version to help the development team test the website before its launch! In addition, a dedicated webpage on antYbuddY.com will be created for you where your name will take permanent presence together with my thanks.

£5 or more

Thank you! You’ll get a personal shout out from me

£15 or more

10 % discount* off a full size Novus Antibody and a freebie1 of your choice (pens, flash drives, luggage tags and seed packets).

£25 or more

20 % discount* off a full size Novus Antibody and a freebie1 of your choice (pens, flash drives, luggage tags and seed packets)

£100 or more,

You will have your photo added as a permanent fixture, on a dedicated webpage on http://antybuddy.com), 20% discount* off full size Novus antibody and a freebie of your choice (pens, flash drives, luggage tags and seed packets)

£3000 (limited to 2 spaces2)

Dedicated space (logo/banner) on the AntyBuddy website for 3 months after beta testing

£5000 (limited to 1 space2)

Dedicated space (logo/banner) on the AntyBuddy website for 6 months after beta testing

£8000 (limited to 1 space2)

Dedicated space (logo/banner) on the AntyBuddy website for 12 months after beta testing

 

*Eligible countries: US, UK, Ireland, Germany, France, Switzerland, Italy, Denmark, Finland, Sweden, Norway, Iceland and the Netherlands

  1. Due to postage restrictions, Freebies are limited to UK residents only. First come first served basis as stock is limited
  2. Preference given based on registration date of donor
jarema malicki
very good idea!
30/08/2016 19:23:01
Giancarlo Barone
Thanks Jarema, I hope to build a website that only hosts Top Notch reviews and that is interactive. I hope to run rewards and promotions for scientists who use the site and submit the independent quality reviews I seek. Thanks Carlo
20/09/2016 11:20:42
Giancarlo Barone
You can also support our antibody camapign by registering with our thunderclap page using the following link : thndr.me/Cq54pD. This will give our camapign message a greater social reach.
20/09/2016 15:06:31
Giancarlo Barone
Hi and thanks to everyone who has so far donated to my antibody crowdfund campaign. If you have already registered it would be great to see you donate to make this a reality. Best Carlo
06/10/2016 10:37:29
Giancarlo Barone
Some of you will have noticed that I have rewards for that pledge to my crowdfund. Please do not let these put you off donating to the antYbuddY crowdfund! These have been generously sponsored by Novus Biologicals. It's important to state that this does NOT effect the Independence of antYbuddY and NEVER will but instead highlights that some antibody companies realise the nature of the problem and want to engage with its customers. I think this brave of Novus Biologicals and is a positive way forward. Indeed, it is my wish to secure antYbuddY.com financial long term future through advertising placements on the site. Importantly, any funds raised like this will be used to reward scientists for the Quality Antibody Reviews they submit with things like Amazon gift vouchers, monthly Prizes and Antibody promotions. Best Wishes Carlo
06/10/2016 10:48:11
Alen Piljic
Great idea! I work in science web development. We made Life Science Network (lifescience.net), Science Simplified (scimplified.com) and Science HR (science.hr). We now work also on external projects. If you are interested, we can develop this for you for much less money than indicated above. If you are interested, let me know (http://www.lifescience.net/profile/alen/) Cheers, Alen
06/10/2016 17:20:03
brazier gregory
Hello I'm BRAZIER Gregory agricultural operator in France, but I opted for the financing of projects with the intention of financially support all serious people with projects. So if you need financing and projects, feel free to contact me. Here is my mail: braziergregory001@outlook.fr
05/01/2017 19:17:25
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